Based on the MICs, the ethyl acetate fraction has the best inhibitory activity. However, the bactericidal effects of the test mushroom were not achieved in the experiment. auricula extract, n-hexane fraction, and ethyl acetate fraction against S. Scanning Electron Microscopy (SEM) was used to observe any morphological changes in the tested bacteria.

Microdilution method was employed to determine the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), which were later compared with the antibiotic properties of tetracycline. auricula extracts and fractions was tested for antimicrobial activity. After a concentrated extract was formed, it was subjected to phytochemical screening and fractionation using liquid-liquid extraction (LLE) with n-hexane, ethyl acetate, and methanol-water solvents. The next step was reflux extraction using 96% ethanol. The simplicia was characterized by its water content, ash content, acid solubility, and total ash content. The first stage of the test comprised the collection, determination, and processing of the test plant. This study aimed to determine the antimicrobial activity of the A. The wood ear mushroom ( Auricularia auricula) has been empirically and scientifically proven as an agent that can treat infections because it contains antimicrobial properties like polysaccharide and protein complex.

Infection is a disease caused by pathogenic bacteria, such as Staphylococcus aureus, Escherichia coli, Bacillus cereus, and Pseudomonas aeruginosa.